1. Mix the 300 ug exosomes from HEK293T with 1.68 nmol 3WJ Nanoparticles in PBS buffer, and incubate at 37 °C for 45 min, then on ice for 1 hr.

2. Run ultracentrifugation at 120,000 g for 70 min,with adding Exojuice as cushion to remove the free RNA nanoparticles. Harvest the layer at the interface of Exojuice for use.

Reference:

1.   Zheng Z, Li Z, Xu C, Guo B, Guo P. Folate-displaying exosome mediated cytosolic delivery of siRNA avoiding endosome trapping. J Control Release. 2019 Oct;311-312:43-49.[][]

2.   Pi F, Binzel D, Lee TJ, Li Z, Sun M, Rychahou P, Li H, Haque F, Wang S, Croce CM, Guo B, Evers BM,Guo P. Nanoparticle orientation to control RNA loading and ligand display on extracellular vesicles for cancer regression. Nature Nanotechnology. 2018 Jan; 13(1):82-89. [][]